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. 2014 Nov 5;42(21):13161–13173. doi: 10.1093/nar/gku1089

Figure 5.

Figure 5.

Mitochondrial DNA repair enzymes are PARylated under oxidative stress conditions. (A) PARylation of mitochondrial-specific DNA repair enzymes, EXOG and Polγcat, is induced by oxidative stress in control but not in PARP1-depleted A549 cells. (B) EXOG, the mitochondrial-specific DNA repair enzyme is PARylated in oxidatively stressed cells. A549 cells were exposed to 0.1 U/ml GOx to induce oxidative stress. Immunoprecipitation (IP) of EXOG from mitochondrial fraction was performed as described in ‘Materials and Methods’. FLAG-specific antibody was used to ensure specificity of the transfection. EXOG and PAR-specific antibodies were used for western analysis. (C) In cellulo PARylation of nuclear-specific BER enzymes, FEN1 and Polβ, in resting and oxidatively stressed of control and PARP1-depleted A549 cells was analyzed by PLA. Representative images of at least two independent experiments are shown.