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. 2014 Nov 6;42(21):13174–13185. doi: 10.1093/nar/gku1095

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Hug1 is an IDP. (A) CD spectra in far-UV region are monitored at several temperatures spanning from 10 to 100°C on purified recombinant Hug1 by using a spectropolarimeter equipped with a water circulation temperature control. (B) Effect of temperature on far UV CD spectra of Hug1. Thermal unfolding transition curves are provided by plotting the change in ellipticity at 197 nm and 222 nm from A across temperature. No melting transition is observed for Hug1. (C) Hug1's apparent R_s(star) based on its elution time in Supplemental Figure S4 matches that of a natively unfolded protein with a PMG-like structure. R_s, Stokes radius; MW, Molecular Weight. Lines corresponding to five known protein conformations were reported according to (28). (D) Chemical shift index calculated for Hug1 ¹³C^α nuclei using random coil shift set from (30). Positive values represent α-structure propensity. Nascent alpha-helix is indicated above.