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. 2014 Nov 11;42(21):13061–13073. doi: 10.1093/nar/gku1124

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Repression in trans by tetTALE-SD. (A) Schematic picture of the reporter construct stably integrated in HAFTL cells to analyze the trans-repression capacity of tetTALE-SD on chromosomal targets (tetEF ZsGreen). The green fluorescent protein ZsGreen serves as reporter. (B) HAFTL tetEF ZsGreen cells before transfection (w/o) and single clones isolated from the tetTALE-SD+ pool were analyzed by immunoblotting for tetTALE-SD expression levels. β-actin levels served as a loading control. (C) Microscopic picture of HAFTL tetEF ZsGreen cells after stable transfection with tetTALE-SD: ZsGreen (left), mCherry (middle), merge (right). Scale bar: 50 μm. (D) FACS analysis of HAFTL tetEF ZsGreen cells before (w/o) and after stable transfection with tetTALE-SD or tetTALE and selected clones originating from the tetTALE-SD transfected pool.