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. 2014 Nov 11;42(21):13206–13213. doi: 10.1093/nar/gku1136

Figure 1.

Figure 1.

Perfect palindrome induced breaks cause death in recombination-proficient cells. (A) Colony formation of 10-fold dilutions of a strain containing the perfect 460 bp palindrome was compared to 10-fold dilutions of a strain without a chromosomal palindrome and of a strain containing a 480 bp interrupted palindrome. In the left panel, cells were incubated in the presence of glucose to repress the expression of SbcCD and in the right panel, cells were incubated in the presence of arabinose to induce the expression of SbcCD. (B) The optical density of the same three strains and their recB mutant derivatives was monitored as a function of time after addition of arabinose to induce the expression of SbcCD. Cells were maintained in exponential phase by dilution and the plotted optical density was adjusted for that dilution. The strains used were DL2792 (lacZ+), DL3020 (lacZ::460 bp), DL3021 (lacZ::480 bp), DL2797 (ΔrecB lacZ+) DL3066 (ΔrecB lacZ::460 bp) and DL3048 (ΔrecB lacZ::480 bp). Error bars represent the standard error of the mean of three biological replicates.