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. 2014 Nov 11;42(21):13228–13241. doi: 10.1093/nar/gku1149

Figure 1.

Figure 1.

Lethal overinitiation is suppressed by the absence of oxygen. (A) The hda::cat allele was introduced into wt and hsm-2 cells by bacteriophage P1 transduction. Plates were incubated for 16 h at 37°C on LB plates supplemented with 0.2% glucose and chloramphenicol aerobically and anaerobically. (B) dnaAcos mutant cells were streaked on LB plates supplemented with 0.2% glucose followed by incubation aerobically at either 42°C (permissive temperature) or 30°C (non-permissive temperature) and anaerobically at 30°C. (C) Wild-type hsm-2 cells were transformed anaerobically with plasmid pBR322-DARS2. Cells from the resultant colonies were subsequently re-streaked aerobically and anaerobically on LB plates supplemented with 0.2% glucose and incubated at 37°C for 16 h.