Table 1.
| Strain | Growth conditiona | Origins/cellb | Cell massc | Origins/massd | Doubling time (min) |
|---|---|---|---|---|---|
| wt | aerobic | 4.7 | 1 | 1 | 33 |
| wt | anaerobic | 4.0 | 0.9 | 1 | 49 |
| wt | 1 h after shift to aerobic growth | 4.7 | 1 | 1 | 33 |
| wt | 2 h after shift to aerobic growth | 4.7 | 1 | 1 | 33 |
| Δhda | anaerobic | 5.9 | 1 | 1.3 | 52 |
| Δhda | 1 h after shift to aerobic growth | 8.8 | 1 | 2 | NR |
| Δhda | 2 h after shift to aerobic growth | 9.6 | 1.2 | 1.8 | NR |
| Δhda | 4 h after shift to aerobic growth | 9.5 | 1.3 | 1.6 | NR |
| wt/ pBR322-DARS2 | anaerobic | 7.4 | 0.9 | 1.7 | 52 |
| wt/pBR322-DARS2 | 3 h after shift to aerobic growth | 11.8 | 1.3 | 2 | NR |
aGrowth was in minimal medium supplemented with glucose and casamino acids.
bDetermined as average fluorescence from flow cytometric analysis.
cDetermined as average light scatter from flow cytometric analysis.
dAverage fluorescence/average light scatter. Numbers are normalized to 1 for wt grown aerobic.
NR, not relevant.