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. 2014 Oct 7;42(21):e161. doi: 10.1093/nar/gku864

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Distribution of means and variances for simulated and real Zebrafish data. To confirm that my simulation procedure produced reasonable simulated counts, I plotted the gene-specific means and variances for (left panel) the simulated data set and (right panel) the observed Zebrafish data set. The two distributions are qualitatively similar. Additional checks on the simulation procedure are provided in the simulated data analysis at http://jtleek.com/svaseq/simulateData.html.