Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Sep 26;42(21):e163. doi: 10.1093/nar/gku869

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Proper nuclear localization of the NLS-tagged proteins in MEL cells. (a–i) Immunofluorescence experiments in MEL/BirA (a, b, c), MEL/BioFli-1 (d, e, f) and MEL/BioIrf2bp2 (g, h, i) cells using either DAPI (a, d, g) or streptavidin conjugated with Alexa fluor 488 (b, e, h). The figure c, f and i show the merged picture. (j and k) Total (lanes 1 and 2) and nuclear (lanes 3 and 4) proteins were extracted from MEL/BirA (lanes 1 and 3), MEL/BioFli-1 (j, lanes 2 and 4) and MEL/BioIrf2bp2 (k, lanes 2 and 4) and subjected to Western blot analysis. Membranes were probed using an antibody against the endogenous protein (top panel) or against Vcp (bottom panel, loading control).