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. 2014 Sep 26;42(21):e164. doi: 10.1093/nar/gku870

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Published by Oxford University Press on behalf of Nucleic Acids Research 2014. This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 4. — Expression of the human BRCA1 gene in porcine ST cells. (a) From hamster CHO cells exhibiting a high efficiency of microcell formation, the alphoid^tetO -HAC/BRCA1 was transferred into the pig's testis fibroblast ST cells using MMCT technique. (b) FISH analysis of porcine cells containing the alphoid^tetO-HAC/BRCA1. Chromosomal DNA was counterstained with DAPI (blue). Arrow indicates to the HAC. (c) Fluorescence image of cells carrying the alphoid^tetO -HAC/BRCA1 containing the expressed eGFP transgene. (d) Expression of the human BRCA1 gene in porcine cells. RT-PCR analysis of genomic DNA isolated from the original ST cells (lane 4), from two porcine clones (pl4 and pl5) containing the alphoid^tetO -HAC/BRCA1 (lanes 2 and 3) and from the human HeLa cells as a positive control (lane 1) using the human BRCA1-specific primers (Supplementary Table S1). The size of the predicted product is 839 bp. M-ladder containing alphoid^tetO -HAC/BRCA1