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Characterization of varying numbers of PCR cycles in SHAPE-Seq library construction. SHAPE-Seq v1.0 libraries were constructed for tRNA and 5S rRNA using either 6×, 9×, 12× or 20× cycles of PCR before sequencing. (+) and (–) fragment distributions for each RNA were compared between 6× cycles and the other cycle numbers as in Figure 2. Pearson's correlation values (R) for individual comparisons between fragment distributions, and {θi} distributions, are shown on the bottom.
