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. 2014 Oct 10;42(21):e165. doi: 10.1093/nar/gku909

Figure 4.

Figure 4.

Schematic of traditional SHAPE/SHAPE-Seq v1.0 RT priming strategies and the universal RT priming strategy of SHAPE-Seq v2.0. Traditional strategies use sequences that are part of the RNA, or added structure cassette flanking sequences to prime RT reactions. In SHAPE-Seq v2.0, a linker sequence is added to the RNA post-modification, which serves as a priming site for the RT reaction (see Supplementary Figure S4).