Table 2. Predicted^aand Experimental^b,cR_h Values (nanometers) and Compaction Factors^d (C_f) for αS, βS, and βS_HC in Various States.

		U	F	NaClb	NaCl and SDSc
αS	Rh	3.70	1.99	3.19	2.46
	Cf		0.61	0.30	0.725
βS	Rh	3.60	1.97	3.24	3.22
	Cf		–	0.22	0.23
βSHC	Rh	3.77	2.01	–	2.97
	Cf		–	–	0.46

^aU and F refer to the R_h values predicted according to eq 7(27) for a fully unfolded and a compact folded polypeptide, respectively.

^bMeasured by PFG-NMR on 200 μM protein in D₂O and 20 mM phosphate buffer (pH 6.5) with 100 mM NaCl at 288 K.¹³ Note that the R_h measured by PFG-NMR for 100 μM αS in unbuffered D₂O at 288 K is almost identical (3.20 nm).¹²⁶

^cMeasured by PFG-NMR on 70 μM protein in 10 mM phosphate buffer (pH 7.7) with 100 mM NaCl and 0.5 mM SDS at 298 K.¹²⁹

^dCalculated according to eq 6.²⁷