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Backbone order parameters (S2) of HbI and F97Y HbI. Order parameters, S2, are shown as a function of residue number for WT HbI (a) and F97Y HbI (b). S2 values calculated for CO-bound HbI are shown as filled circles, and S2 values for unliganded HbI are shown as empty circles. Secondary structural elements are shown at the top of each plot. Solid bars represent α-helices, and lines represent loops.
