Table 1. Measured Strand Displacement Rate Constants of S by f (in units of M^–1 s^–1)^a.

n	kf of S (25 °C)	kf of S (41 °C)
10	121000	228000
8	96100	188000
7	80600	148000
6	77100	102000
5	55200	60000
4	1790	2890
3	1210	1680
2	13.1	23.1
1	0.92	16.5
0b	N/A	2.10b

^aThe concentration of [S] = 3 nM, [f] = 30 nM (except for n = 4, [f] = 100 nM, n = 3, [f] = 1 μM, n = 2, [f] = 10 μM, and for n = 1, [f] = 100 μM).

^bThe value for n = 0 was measured at 37 °C.