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Pyrene fluorescence assay of asymmetric amphiphiles. The amphiphile consisting of an A160 ELP and a (YGG)8, assembly domains does not self-assemble and shows no concentration dependence in its I1/I3 ratio, whereas amphiphiles with (FGG)8 and (WGG)8, assembly domains self-assemble into cylindrical micelles and show a two-state concentration-dependent behavior in their I1/I3 ratio. The (FGG)8 core is more hydrophobic than the (WGG)8 core, as indicated by its lower final I1/I3 value.
