Cryo-TEM micrographs
of genetically encoded asymmetric amphiphiles.
(A–C) A160-(YGG)8, A160-(YG)8, and A160-Y8, respectively. (A) Constructs such as A160-(YGG)8 that do not self-assemble could not be visualized by cryo-TEM because
of their high levels of hydration and low densities. (B,C) Changing
the assembly domain from (YG)8 (B) to Y8 (C)
causes a significant decrease in the length of the cylindrical micelles.
(D–F) A160-(FGG)8 (D), A80-(FGG)8 (E),
and A40-(FGG)8 (F) self-assemble into cylindrical micelles
with similar aspect ratios. Scale bar represents 200 nm.