Table 2. Amphiphile Characterization by Light Scattering and Tunable Resistive Particle Sizing.
amphiphile | MW g·mol–1 | Nagga | Rgb (nm) | Rhc (nm) | Rd (nm) | ρe |
---|---|---|---|---|---|---|
A160-(IGG)8 | n.d.f | n.d.f | n.d.f | 6.5 ± 0.8 | n.d.f | n.d.f |
A160-(LGG)8 | n.d.f | n.d.f | n.d.f | 6.1 ± 0.7 | n.d.f | n.d.f |
A160-(HGG)8 | n.d.f | n.d.f | n.d.f | 6.7 ± 0.7 | n.d.f | n.d.f |
A160-(YGG)8 | n.d.f | n.d.f | n.d.f | 6.6 ± 0.8 | n.d.f | n.d.f |
A160-(FGG)8 | 6.89 × 106 | 111 | 55.93 | 42.8 ± 9.9 | 53.5 ± 4.8 | 1.31 |
A160-(WGG)8 | 2.64 × 107 | 426 | 110.5 | 80.6 ± 35.6 | 76.0 ± 12.1 | 1.37 |
A160-(YG)8 | 2.47 × 107 | 399 | 100.0 | 72.7 ± 13.1 | 71.0 ± 8.9 | 1.37 |
A160-Y8 | 3.36 × 106 | 53 | 45.0 | 57.85 ± 1.5 | n.d.g | 0.78 |
A160-(FG)8 | 4.68 × 106 | 76 | 46.1 | 33.0 ± 4.9 | 54.5 ± 5.5 | 1.40 |
A160-F8 | 3.60 × 107 | 572 | 124.4 | 91.4 ± 9.7 | 73.0 ± 6.3 | 1.36 |
A80-(FGG)8 | 9.00 × 106 | 290 | 47.87 | 35.3 ± 4.3 | 55.5 ± 6.0 | 1.36 |
A40-(FGG)8 | 8.62 × 106 | 575 | 44.32 | 30.9 ± 2.6 | 54.5 ± 5.4 | 1.43 |
The aggregation number Nagg, or the number of polypeptide chains per nanoparticle, was determined by static light scattering
Rg, radius of gyration, was determined by static light scattering.
Rh, hydrodynamic radius, was determined by dynamic light scattering.
R was determined by tunable resistance pulse sensing.
ρ represents the shape factor and is equal to Rg/Rh.
n.d. indicates no data: static light scattering and TRPS measurements were not performed for nonself-assembling amphiphiles.
No data: we were unable to measure the size of A160-Y8 by TRPS.