Table 2. Amphiphile Characterization by Light Scattering and Tunable Resistive Particle Sizing.
| amphiphile | MW g·mol–1 | Nagga | Rgb (nm) | Rhc (nm) | Rd (nm) | ρe |
|---|---|---|---|---|---|---|
| A160-(IGG)8 | n.d.f | n.d.f | n.d.f | 6.5 ± 0.8 | n.d.f | n.d.f |
| A160-(LGG)8 | n.d.f | n.d.f | n.d.f | 6.1 ± 0.7 | n.d.f | n.d.f |
| A160-(HGG)8 | n.d.f | n.d.f | n.d.f | 6.7 ± 0.7 | n.d.f | n.d.f |
| A160-(YGG)8 | n.d.f | n.d.f | n.d.f | 6.6 ± 0.8 | n.d.f | n.d.f |
| A160-(FGG)8 | 6.89 × 106 | 111 | 55.93 | 42.8 ± 9.9 | 53.5 ± 4.8 | 1.31 |
| A160-(WGG)8 | 2.64 × 107 | 426 | 110.5 | 80.6 ± 35.6 | 76.0 ± 12.1 | 1.37 |
| A160-(YG)8 | 2.47 × 107 | 399 | 100.0 | 72.7 ± 13.1 | 71.0 ± 8.9 | 1.37 |
| A160-Y8 | 3.36 × 106 | 53 | 45.0 | 57.85 ± 1.5 | n.d.g | 0.78 |
| A160-(FG)8 | 4.68 × 106 | 76 | 46.1 | 33.0 ± 4.9 | 54.5 ± 5.5 | 1.40 |
| A160-F8 | 3.60 × 107 | 572 | 124.4 | 91.4 ± 9.7 | 73.0 ± 6.3 | 1.36 |
| A80-(FGG)8 | 9.00 × 106 | 290 | 47.87 | 35.3 ± 4.3 | 55.5 ± 6.0 | 1.36 |
| A40-(FGG)8 | 8.62 × 106 | 575 | 44.32 | 30.9 ± 2.6 | 54.5 ± 5.4 | 1.43 |
a
The aggregation number Nagg, or the number of polypeptide chains per nanoparticle, was determined by static light scattering
b
Rg, radius of gyration, was determined by static light scattering.
c
Rh, hydrodynamic radius, was determined by dynamic light scattering.
d
R was determined by tunable resistance pulse sensing.
e
ρ represents the shape factor and is equal to Rg/Rh.
f
n.d. indicates no data: static light scattering and TRPS measurements were not performed for nonself-assembling amphiphiles.
g
No data: we were unable to measure the size of A160-Y8 by TRPS.