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. 2014 Oct 15;8(11):11243–11253. doi: 10.1021/nn503732m

Figure 5.

Figure 5

In vitro PLN wound adhesion and platelet aggregation in simulated wound environment. (a) Experimental setup for flowing PLNs and activated platelets together over VWF–collagen-coated surfaces. PLNs first encounter a BSA region so as to limit nonspecific binding at the collagen–VWF-coated surface. (b) PLNs are able to adhere to VWF–collagen-coated surfaces while aggregating with natural platelets in a PPFC better than PLNs with no peptides, FMP-only, or CBP + VBP-only. Representative images are shown for the end of the experiment (30 min) for each condition. Scale bar = 50 μm. All images were taken at the same magnification. (c) Pearson’s colocalization coefficient values for: (i) activated platelets + PLNs without peptides (white bars), (ii) activated platelets + PLNs with CBP + VBP modification (hatched bars), (iii) activated platelets + PLNs with FMP modification (gray bars), and (iv) activated platelets + PLNs with CBP + VBP + FMP modification (black bars). At least 10 images for each condition were used for analysis. *Denotes statistical difference (P < 0.05) from all other groups.