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. Author manuscript; available in PMC: 2014 Nov 28.
Published in final edited form as: Science. 2014 May 2;344(6183):527–532. doi: 10.1126/science.1252651

Figure 3. Remodelin targets NAT10 to improve nuclear shape and fitness of HGPS cells.

Figure 3

A) Representative immunofluorescence (IF) pictures of Lamin A/C in HGPS cell lines compared to matched normal fibroblasts at the same population doubling. Scale bar: 10 μm. B) Quantification of misshapen nuclei upon Remodelin treatment (means of three independent experiments with n>213 ± s.e.m.). C) Lamin A/C staining in HGPS AG11498 cells (left) and quantification of misshapen nuclei (right; means of three independent experiments with n>176 ± s.e.m.). Scale bar: 50 μm. D) Western blotting analysis of γH2AX after Remodelin or FTI treatment. E) Immunofluorescence analysis of γH2AX staining upon Remodelin or ATM/ATR inhibition (ATM/ATRi). F) Quantification of γH2AX positive cells observed by IF (means of three independent experiments with n>127 ± s.e.m.). G) HGPS proliferation upon Remodelin or ATM/ATR-inhibitor treatment (means of nine replicates ± s.e.m). H) Quantification of senescence-associated β-galactosidase positive cells (means of three independent experiments with n>257 ± s.e.m.). I) Quantification of senescence-associated β-galactosidase positive cells in HGPS AG11498 after 8 days of Remodelin treatment at population doubling 12 (PDL 12) and after several weeks of Remodelin treatment and 12 cell divisions (PDL 24) (means of two independent experiments with n>298 ± s.d.).