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Separation of subfractions of the cross-linked PC-APC by two-dimensional gel electrophoresis (protocol 3). A, analysis of non-cross-linked PBSs shows the absence of cross-linked products with lower mobility in the second dimension. B, spots 1–5 appear following cross-linking with GA. C, spots 1–5 appear following cross-linking with BS3. Spots produce fluorescence when scanned using the Cy3 and Cy5 laser/filter sets. Red, PC; green, APC.
