FIGURE 6.

Recognition of prM proteins of WT and MH domain mutants on VLPs by different anti-prM mAbs based on dot blot binding assay. A, at 48 h, pellets (containing VLPs) derived from ultracentrifugation of culture supernatants of 293T cells transfected with WT or mutant constructs (in 1× PBS) were dotted on nitrocellulose membrane and subjected to dot blot binding assay using six anti-prM mAbs and mixed mAbs. The layout and controls of the assay are presented as in Fig. 2D. B, the recognition index (R.I.) of each mAb to mutant prM protein as described under “Experimental Procedures” (46, 47) is shown in gray bars. The data are means with standard deviation from two experiments.