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Localization of ERK1/2 in a human adipocyte. Adipocytes were immunostained with fluorescent antibodies against ERK1 (green fluorescence), stained for nuclei with DAPI (blue fluorescence) (A and C), and examined by fluorescence confocal microscopy. One cell is shown, with a single plane through the nucleus (A and B) and a three-dimensional stack of the perimeter of the cell containing the nucleus (C and D). A scale bar is also shown.
