FIGURE 2.

Effect of STAM mutants on Hrs subcellular localization and endosomal morphology in Hrs-overexpressing cells. A, schematic representation of the structure of the STAM1 deletion mutants. The Vps27-Hrs-STAM (VHS), UIM, Src-homology 3 (SH3), and coiled-coil domains are indicated at the top of the diagram. B, expression of STAM1 mutants lacking the SH3 domain or the coiled-coil region fail to induce a diffuse localization of Hrs. HEK293T cells were transiently transfected with HA-Hrs and either an empty vector (mock), wild-type STAM1, or STAM1 deletion mutants and then immunostained with anti-Hrs and anti-STAM1 antibodies. Scale bars, 5 μm.