FIGURE 4.

Phosphorylation-coupled conformational change enables ezrin-Stx3 interaction. A, representative molecule structures of Stx3. B, statistic histogram of single molecule mean height of Stx3. The major peak value is 0.32 nm from a total of 120 measurements of three independent preparations. C, representative molecule structures of Stx3 mixed with ezrin^S66D. Bar, 1 μm. D, representative molecule structures of Stx3 mixed with ezrin^S66D from a different preparation. Bar, 1 μm. E, magnified single molecule structure of Stx3 incubated with ezrin^S66D. Bar, 100 nm. F, statistic histogram of single molecule height of Stx3 incubated ezrin^S66D. There three major peak mean values: 0.34 nm (Stx3 alone), 0.59 nm (ezrin^S66D alone), and 0.91 nm (Stx3-bound ezrin^S66D). Representative magnified images were also shown from a total of 145 measurements of three independent preparations.