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Flippase activities of P4-ATPases at 4 °C. HeLa cell lines stably expressing the HA-tagged ATP11A(clone 14) and ATP8B1(clone 12) were incubated with NBD-PS (A), NBD-PE (B), or NBD-PC (C) at 4 °C for the indicated times. The graph displays averages from two independent experiments ±S.D. Error bars represent S.D.
