TABLE 2.
NGS and Sanger sequencing results for in vivo and in vitro derived antibodies
The number of samples, reads, and indels observed for in vivo and in vitro derived antibody samples is reported. The number of in-frame (e.g., n = 3,6,9, … ) indels observed is shown in parentheses. NA means not available.
| In vitro AID, no binding selection | In vitro no AID | In vitro AID, binding selection | In vivo | |
|---|---|---|---|---|
| HC | ||||
| Samples | 18 | 3 | 132 | 68 |
| Reads | 1,672,921 | 363,535 | 143,329 | 285,331 |
| Indels | 274 (113) | 0 | (54) | 1,497 (1,173) |
| LC | ||||
| Samples | 16 | NA | 132 | 1 |
| Reads | 1,419,998 | NA | 118,762 | 197,374 |
| Indels | 215 (91) | NA | (51) | 899 (658) |