FIGURE 2.

VP40 forms intraluminal vesicles and buds effectively into PS containing GUVs. A, POPC:POPS (24:1) and POPC:POPS (4:1) in GUV containing 0.1% LRPE were imaged without VP40 in PBS buffer, which lead to no detectable vesicle formation. B, incubation of the GUV with 5 μm VP40 for 20 min lead to intraluminal budding of vesicles into the GUV in PS-rich GUVs (20% PS, POPC:POPS 4:1), which is not evident in the low PS containing GUVs (4% PS). C, to diminish the effect of VP40 in the PS-rich GUVs so as to increase the ability to visualize and quantify intraluminal budding, the GUVs were incubated with 500 nm VP40. Scale bars = 10 μm.