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. 2014 Oct 9;289(48):33629–33643. doi: 10.1074/jbc.M114.589556

FIGURE 2.

FIGURE 2.

RNase L directly regulates SRF mRNA to down-regulate TTP transcription following mitogen stimulation. A, TTP primary transcripts were measured at the indicated times post-mitogen stimulation of WT and RNase L KO MEFs by qRT-PCR using intronic primers to detect unspliced transcripts. Bars represent the mean ± S.D. of four independent experiments normalized to HPRT mRNA. B, SRF mRNA was measured at the indicated times post-mitogen stimulation of WT and RNase L KO MEFs by qRT-PCR. Bars represent the mean ± S.D. of five independent experiments normalized to HPRT mRNA. C, SRF mRNA decay kinetics were measured by actD time courses in WT and RNase L KO MEFs at 1-h post-mitogen stimulation. SRF mRNA half-lives and S.D. shown were calculated from the indicated number of independent experiments; representative data from one experiment are shown (mean ± S.D. of three independent samples) (p < 0.001). D, presence of SRF mRNA in a complex with RNase L was analyzed by RNP-IP as described in Fig. 1C. Bars represent mean ± S.D. of three independent experiments; *, p < 0.05.