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. 2014 Oct 15;289(48):33644–33654. doi: 10.1074/jbc.M114.609800

FIGURE 3.

FIGURE 3.

Hsp90/ATP binds unfolded CTA1 and is not released after CTA1 refolding. A, Hsp90/ATP was perfused over a CTA1-coated SPR sensor at 15 °C (dashed line) or 37 °C (solid line). The arrowhead denotes removal of Hsp90/ATP from the perfusion buffer. B, Hsp90/ATP was perfused at 15 °C over a SPR sensor coated with heat-denatured CTA1. The arrowhead denotes removal of Hsp90/ATP from the perfusion buffer. C, Hsp90/ATP was perfused over a CTA1-coated SPR sensor at 37 °C and was removed from the perfusion buffer after 300 s (arrowhead). This was followed by a stepwise temperature decrease to 15 °C, which was held for 10 min, before returning the temperature to 37 °C. Upon return to 37 °C, the continued association of Hsp90 with CTA1 was verified by the addition of an anti-Hsp90 antibody to the perfusion buffer (arrow). Note that the time scale for panel C is distinct from the time intervals in panels A and B.