FIGURE 8.
Clathrin- and AP-2-mediated internalization of D2-WT. HEK293 cells stably expressing D2-WT were transfected with GRK2 and either pcDNA3.1, wild type arrestin3 (Arr3-WT), or arrestin3 mutants: Arr3-ΔLIEFD, Arr3-R395E, or Arr3-ΔLIEFD/R395E. Cells were treated with quinpirole (10 μm) for 30 min, and internalization was assessed using the whole-cell binding technique. Each bar represents the mean ± S.E. (error bars) of five independent experiments. **, p < 0.01; ***, p < 0.001 compared with Arr3-WT, Tukey's multiple comparison test.