FIGURE 2.

ADAM8 induction by hypoxia and dimerization are differentially regulated in ERα-negative versus -positive breast cancer cells. A and B, indicated ERα-negative (A) or ERα-positive (B) breast cancer lines were cultured under normoxic (Normox.) or hypoxic (Hypox.) (1% O₂) conditions for 24 h. WCEs were isolated and samples before (−) and after (+) treatment with PNGase F subjected to Western blotting for ADAM8, as above. C, samples of WCEs (50 μg) from MCF-7 and MDA-MB-231 cells before (−) or following (+) BS3 cross-linking were compared using Western blotting for ADAM8 and for β-tubulin. The white dashed line indicates the migratory position of the proform in ERα-negative MDA-MB-231 cells. MDA-231, MDA-MB-231.