FIGURE 7.

N91Q and N612Q mutant ADAM8 proteins are retained in the Golgi and ER, respectively. A and B, HEK-293 cells were transiently transfected with vectors expressing WT ADAM8 or N-glycosylation mutants N91Q and N612Q. Cells were fixed, permeabilized, and subjected to immunofluorescent staining using (A) ADAM8 LSBio antibody (red), M6PR (Golgi marker) (green), and DAPI (blue) or (B) Myc antibody (green); PDI (ER marker) (red) and DAPI (blue). Images were taken as described in Fig. 6A. Scale bar, 10 μm.