Table 1.
List of proteins differently expressed under drought priming analysed by MALDI-MS and MS/MS
| Spot no. | FCa | Protein name | Accession no. | Taxonomy | Theor. M r b/pI | Exp. M r c/ pI | Match no.d | SCe | E-value | Peptides sequence | Function |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Upregulated under drought priming | |||||||||||
| 403 | 6.1 | Rubisco large chain | gi|14017580 | Triticum aestivum | 53.4/6.2 | 63/6.6 | 39 | 63 | 1.3E–09 | Photosynthesis | |
| 688 | 1.5 | Predicted: peptidyl-prolyl cis- trans isomerase CYP38 | gi|357147646 | Brachypodium distachyon | 46.5/4.8 | 46/4.7 | 13 | 30 | 6.5E–07 | Photosynthesis | |
| 2550 | 1.5 | 4-nitrophenylphosphatase | gi|226491816 | Zea mays | 39.5/5.5 | 35/5.1 | 13 | 26 | 4.1E–05 | Unknown | |
| 2712 | 1.8 | No match | |||||||||
| 2477 | 1.5 | No match | |||||||||
| Downregulated under drought priming | |||||||||||
| 2664 | 1.8 | Rubisco small chain PW9 | gi|132099 | Triticum aestivum | 19.8/8.5 | 17/5.8 | 7.0E–03 | EYPDAYVR | Photosynthesis | ||
| 1.7E–05 | EHNSSPGYYDGR | ||||||||||
| 2726 | 1.5 | Photosystem II stability/ assembly factor HCF136 | gi|357117071 | Brachypodium distachyon | 37.0/5.4 | 43/5.3 | 10 | 30 | 3.2E–03 | Photosynthesis | |
| 2637 | 1.5 | Thioredoxin-like protein CDSP32 | gi|15222954 | Arabidopsis thaliana | 34.0/8.7 | 36/5.5 | 3.2E–04 | GELIGEILR | Stress defence | ||
| 2478 | 1.9 | Putative myosin heavy chain | gi|20197623 | Arabidopsis thaliana | 86.2/4.9 | 34/5.1 | 14 | 15 | 1.5E–02 | Cell structure | |
| 448 | 1.5 | No match | |||||||||
aThe value represents the highest fold change of stem elongation-primed plants (NP) and twice-primed plants (PP) compared with non-primed plants (NN).
bTheoretical M r/pI (kDa).
cExperimental M r/pI (kDa) values were calculated using the Progenesis Samespot software.
dMatch no. represents the number of identified peptides that can be matched with protein peptides in the database.
eSequence coverage (%).