Table 2.

List of proteins differently expressed under drought stress analysed by MALDI-MS and MS/MS

Spot no.	FCa	Protein name	Accession no.	Taxonomy	Theor. M r b/pI	Exp. Mrc/pI	Match no.d	SCe	E-value	Peptides sequence	Function
Upregulated under drought stress
3180	1.8	Oxygen-evolving enhancer protein 1	gi|357111487	Brachypodium distachyon	34.8/5.7	35/5.2	10	39	3.3E–07		Photosynthesis
3621	1.6	Ascorbate peroxidase	gi|226897533	Triticum aestivum	26.8/5.5	32/5.3	11	38	5.2E–05		Stress defence
3646	1.6	2-Cys peroxiredoxin BAS1	gi|2499477	Hordeum vulgare	23.4/5.5	28/5.2	6	32	5.1E–04		Stress defence
3010	2.7	Plastid glutamine synthetase isoform GS2c	gi|71362640	Triticum aestivum	47/5.8	43/5.1	6	17	8.4E–03		Protein synthesis
Downregulated under drought stress
3600	1.5	Fructose-bisphosphate aldolase 2	gi|326499908	Hordeum vulgare	41.8/6.4	39/5.6	18	38	5.2E–08		Metabolism
3572	2.3	Fructose-bisphosphate aldolase, cytoplasmic isozyme 1-like	gi|326493652	Hordeum vulgare	38.1/6.1	42/6.7	9	21	2.1E–08		Metabolism
833	2.2	Fructose-bisphosphate aldolase, cytoplasmic isozyme 1-like	gi|326523629	Hordeum vulgare	41.6/7.1	42/6.2	7	11	1.4E–02		Metabolism
3023	2.5	Chloroplast fructose-bisphosphate aldolase	gi|223018643	Triticum aestivum	42.2/5.9	46/5.6	17	50	5.2E–12		Metabolism
3235	1.3	Fructose-bisphosphate aldolase, cytoplasmic isozyme 1-like	gi|326499908	Hordeum vulgare	41.7/6.4	37/5.7	17	38	3.7E–02		Metabolism
3493	1.7	Triosephosphate-isomerase	gi|326496613	Hordeum vulgare	32.7/7.0	30/5.8	9	28	6.4E–03		Metabolism
850	1.9	Cytosolic malate dehydrogenase	gi|37928995	Triticum aestivum	24.6/6.6	41/6.3	6	23	1.3E–02		Metabolism
3042	1.5	Phosphoglycerate kinase	gi|129915	Triticum aestivum	50/6.6	48/5.6			4.7E–05	SVGDLTAADLEGKR	Metabolism
3323	1.9	Rubisco small subunit	gi|11990901	Triticum aestivum	19.7/8.8	16/6.3	9	50	2.1E–05		Photosynthesis
3068	2.5	Rubisco activase small isform	gi|313574196	Hordeum vulgare	47.3/7.6	42/6.4	15	38	5.1E–10		Photosynthesis
3109	2.2	Rubisco activase B	gi|7960277	Triticum aestivum	48/6.9	45/5.7	12	34	2.9E–03		Photosynthesis
3198	1.8	Rubisco activase	gi|37783283	Triticum aestivum	22.5/5.0	47/6.1	11	51	1.3E–10		Photosynthesis
3111	2.4	Rubisco activase alpha form precursor	gi|32481061	Deschampsia antarctica	51.4/6.0	45/5.7	23	44	5.1E–11		Photosynthesis
3214	2.8	Rubisco activase alpha form precursor	gi|32481061	Deschampsia antarctica	51.4/6.0	50/5.4	16	37	1.6E–09		Photosynthesis
3016	2.5	Rubisco activase alpha form precursor	gi|32481061	Deschampsia antarctica	51.4/6.0	50/5.6	12	21	4.1E–07		Photosynthesis
3443	1.7	Rubisco activase	gi|37783283	Triticum aestivum	22.5/5.0	42/6.1	7	24	9.9E–04		Photosynthesis
3091	1.5	Rubisco activase small isoform	gi|313574196	Hordeum vulgare	47.3/7.6	49/5.7	16	42	2.9E–03		Photosynthesis
3117	1.6	Actin	gi|168472715	Lolium temulentum	41.2/5.5	51/5.7	12	31	2.1E–10		Cell structure
3133	2.3	Rubisco large subunit-binding protein subunit beta	gi|2493650	Secale cereale	53.7/4.9	61/5.5	12	26	6.6E–08		Molecular chaperones
3149	1.6	Plastid glutamine synthetase isoform GS2c	gi|71362640	Triticum aestivum	47/5.8	49/5.3	19	47	3.3E–11		Protein synthesis
3394	1.9	Cysteine synthase	gi|585032	Triticum aestivum	34.2/5.5	36/6.3	11	32	5.2E–05		Protein synthesis
3131	2.6	Cysteine synthase	gi|585032	Triticum aestivum	34.2/5.5	37/6.1	11	31	2.6E–06		Protein synthesis
3457	1.8	Cysteine synthase	gi|585032	Triticum aestivum	34.2/5.5	37/5.8	13	46	1.6E–06		Protein synthesis
3648	1.7	29kDa ribonucleo protein	gi|326510421	Hordeum vulgare	29.6/4.8	33/4.6			1.6E–02	VNSGPPPPRDEFAPR	Unknown
3339	1.6	Hypothetical protein MTR_026s0001	gi|358343995	Medicago truncatula	12.7/10	41/6.1	6	54	2.1E–03		Unknown

^aThe value represents the highest fold change of non-primed plants under drought stress (NND), stem elongation-primed plants under drought stress (NPD) and twice-primed plants under drought stress (PPD) compared with control (NNC).

^bTheoretical Mr/pI (kDa).

^cExperimental Mr/pI (kDa) values were calculated using the Progenesis Samespot software.

^dMatch no. represents the number of identified peptides that can be matched with protein peptides in the database.

^eSequence coverage (%).