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. 2014 Sep 9;65(22):6529–6542. doi: 10.1093/jxb/eru370

Fig. 3.

Fig. 3.

Average PSII efficiency (ΦPSII) (A) and representative images of ΦPSII (B) for Col-0 leaf discs in response to ABA after prior exposure to different VPDs. ΦPSII was measured under non-photorespiratory conditions (20 mmol mol–1 O2, 380 µmol mol–1 CO2 and remainder N2) in plants that had been exposed for 4 d to moderate (1.17 kPa; black bars in (A)) or to low (0.23 kPa; L; white bars in (A)) VPD in response to ABA. At the end, an image was made after 5min exposure to 20 mmol mol–1 O2 and 50000 µmol mol–1 CO2 (grey bars for M, cross-hatched bars for L in Fig. 3A and +CO2 in Fig. 3B). Leaf discs (0.5cm diameter) were put with the abaxial surface up in petri dishes filled with stomata-opening medium with different concentrations of ABA (0, 50, 100, 200 µM ABA), and ΦPSII was recorded 3h after application of the ABA. Bars represent the mean of ΦPSII of eight leaf discs±standard error of the mean.