Fig. 4.
Effect of PDC2_E1α silencing on C. reinhardtii after 2 d of cultivation under nitrogen starvation in HSM-N (A, C, E, G) and in TAP-N (B, D, F, H) medium. (A, B) Biomass (DW) content. (C, D) Culture Chl content. (E, F) TFA content of biomass. (G, H) Culture TFA content. Values represent means±SD of n=6 for pdh lines (three biological and two technical replicates) and n=12 for EV control lines (joint results of two different control lines). Differences between controls and mutants are significant at P<0.05 (Student’s t-test).