Fig. 3.

The Atcesa1 ^F954L mutation does not rescue the short root phenotype of the Atcesa6 ^prc1 Atcesa1 ^rsw1 YFP–CESA6 mutant. Seedlings were grown for 5 d at a permissive temperature (23 °C), and then transferred to a restrictive temperature (31 °C) for 7 d. After the rsw1 mutation in Atcesa6 ^prc1 Atcesa1 ^rsw1 YFP–CESA6 was rescued with wild-type CESA1, root length was similar to Col-0 because wild-type CESA1 replaced the rsw1 mutant protein. As expected, Atcesa1 ^rsw1 roots were short, as were roots of Atcesa6 ^prc1 Atcesa1 ^rsw1 YFP–CESA6 seedlings that contained only the rsw1 allele of AtCESA1. Two independent Atcesa6 ^prc1 Atcesa1 ^rsw1 YFP–CESA6 lines in which Atcesa1 ^F954L was expressed (Atcesa6 ^prc1 Atcesa1 ^rsw1 YFP–CESA6 Atcesa1 ^F954L-1 and -2) also had short roots, indicating that AtCESA1 is non-functional when the F954L mutation is present. Total root length was measured, error bars represent standard deviation, and asterisks indicate significant differences (P<0.0001, t-test) compared with wild-type Col-0.