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. 2014 Oct 7;65(22):6723–6733. doi: 10.1093/jxb/eru397

Fig. 5.

Fig. 5.

Peroxisomal KAT5 complements kat2 kat5 double knockouts. (A) Western blot of kat mutants. A KAT2 antibody (Germain et al., 2001) was used to probe protein extracted from 7-d-old whole seedlings that had been germinated on 0.5× MS media supplemented with 1% sucrose (left panel) or 2,4-DB (right panel, to select for double knockouts). α-TUB=α-tubulin loading control. The inset depicts resolution of a second, larger band originating in the KAT2 over-expressing line (see text). (B) Restoration of fertility in kat2 kat5 double knockouts by peroxisomal KAT5 (KAT5px). kat2 kat5 phenotypes were rescued by constitutive expression (by CAMV 35S promoter) of KAT5px, but not by a variant of the protein targeted to the cytosol (KAT5cyt). Plants were grown in long-day conditions for 35 d. (C) Complementation of the sucrose-dependent phenotype of kat2 kat5 double knockout. Seedlings were grown on 0.5× MS without sucrose supplement in the dark or in the light for 8 d. kat2-1/kat2-1 kat5-2/KAT5 sesquimutants were unable to establish on media lacking sucrose. The introduction of constitutively expressed KAT5px, but not KAT5cyt, allowed both the recovery of kat2 kat5 double knockouts and their establishment in the absence of sucrose. Scale bar, 20mm. For all panels, the kat2 kat5 mutant (DKO) was kat2-1 kat5-2.