Table 2.
Segregation analysis of kat2-1 kat5-2 mutants homozygous at one locus but segregating at the otherSegregating mutant seedlings were genotyped by PCR to determine their segregation ratio, and this was compared with the expected 1:2:1 ratio for a single recessive mutation by χ2 analysis. The frequency (%) of each genotype is given in brackets.
| Media | Background mutation | Segregating mutation | Total | χ2 (1:2:1 hypothesis) | ||
|---|---|---|---|---|---|---|
| Wild-type | Heterozygous | Homozygous | ||||
| 0.5× MS+1% suc | kat5-2 | KAT2/KAT2 | kat2-1/KAT2 | kat2-1/kat2-1 | ||
| 42 (46%) | 40 (44%) | 9 (9.9%) | 91 | 3.3×10–6 | ||
| 0.5× MS+1% suc | kat2-1 | KAT5/KAT5 | kat5-2/KAT5 | kat5-2/kat5-2 | ||
| 28 (30%) | 46 (49%) | 19 (20%) | 93 | 0.42 | ||
| Water–agar | kat2-1 | KAT5/KAT5 | kat5-2/KAT5 | kat5-2/kat5-2 | ||
| 50 (28%) | 105 (58%) | 26 (14%) | 181 | 0.0041 | ||