Figure 3.

Assessment of the butyrate effect on GPx4 protein expression in VSMC. Proliferating VSMCs were exposed to different concentrations of butyrate for 48 h (A) or treated with 5 mM butyrate for the required periods of time (B). At the end of the treatment, cell lysates were prepared and processed to determine GPx4 protein expression by western analysis, as described in the Experimental Section. Band intensities were measured, normalized and quantitated, as described in legends to Figure 1. The results shown are representative of three independent experiments (top) and the data obtained from three independent experiments are presented as the relative fold difference compared to the untreated control (Con). * p < 0.01 versus untreated VSMC and ** p < 0.001 versus untreated VSMC.