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. 2014 Nov 10;6(11):4280–4293. doi: 10.3390/v6114280

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Expression of SOCS3 in Raw264.7 cells infected with wild-type or inactivated JEV. (A) Quantitative RT-PCR was performed with RNA extracted from mock-infected, inactivated JEV or wild-type JEV-infected RAW264.7 cells after different time points, post-infection. Results were expressed as the fold change in relative mRNA expression over the control. (B) Expression of SOCS3 protein in mock-infected (C), inactivated (IJ) or wild-type JEV-infected (J) RAW264.7 cells was analyzed by Western blotting. Data are expressed as the mean ± SD from four independent experiments, and significance was evaluated by GraphPad Prism 5 software. Differences were assessed by using unpaired Student’s t-tests. ** p < 0.01, *** p < 0.005.