Table 1.

Round diversity and screening coverage

Round	Library size*	Variants screened†	Percent coverage	Beneficial mutations	Libraries
1	4,460	3,780	57.2	84	Saturation mutagenesis
2	128,768	2,352	1.8	25	Combinatorial
3	86,144	2,352	2.7	22	Combinatorial
4	45,920	2,772	5.9	12	Combinatorial
5	4,460	3,948	58.7	14	Saturation mutagenesis
6	19,456	2,016	9.8	9	Combinatorial
7	4,460	3,780	57.2	20	Saturation mutagenesis
8	46,592	2,016	4.2	10	Combinatorial
9	69,996	4,200	5.8	13	Saturation mutagenesis/combinatorial

^*The library size number is a sum of the size of all of the libraries constructed and screened during that round.

^†Randomly selected variants screened for all of the libraries constructed during that round.