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. 2014 Nov 3;111(46):16520–16525. doi: 10.1073/pnas.1415178111

Fig. 6.

Fig. 6.

APC depletion or c-Myc induction triggers BID activation and BID-dependent apoptosis in NSAID-treated normal colonic epithelial cells. (A) NCM356 normal colonic epithelial cells were transfected with the indicated siRNA and then treated with 120 µM sulindac sulfide for 48 h. Indicated proteins were analyzed by Western blotting. C Casp 3, cleaved caspase 3. (B) NCM356 cells were transfected with indicated siRNA or siRNA/plasmid combinations, and then treated with 120 µM sulindac sulfide for 48 h. Apoptosis was measured by counting apoptotic nuclei after nuclear staining. (C and D) Western blotting of the indicated proteins in NCM356 cells transfected as indicated, and then treated with 120 µM sulindac sulfide for 36 h. (E) Apoptosis in NCM356 cells transfected as indicated and treated with 120 µM sulindac sulfide for 24 h was measured as in B. (F) A model of NSAID-mediated apoptosis in APC-deficient cells. Results in B and E were expressed as means + SD of three independent experiments. **P < 0.01; ***P < 0.001; N.S., not significant.