Fig. 1.
Accumulation of STING in Vero cells exposed to the wild-type virus made in HEp-2 cells. Vero cells seeded in 6-well plates were either mock-infected, exposed (10 pfu/cell) to HSV-1(F) virus propagated in HEp-2 cells, or to the ΔICP0 mutant virus propagated in U2OS cells. The cells were harvested at 30 min or at 1, 2, 3, 4, 5, 6, 8, or 18 h after exposure to the viruses. Approximately 60 μg of total proteins per sample were subjected to electrophoresis in a 10% (vol/vol) denaturing polyacrylamide gel, transferred to a nitrocellulose sheet, and immunoblotted with the ICP0 rabbit polyclonal antibody or with the STING, US11, and β-actin mouse monoclonal antibodies. The STING protein was visualized with ECL detection reagent, whereas the ICP0, US11, and β-actin proteins were visualized with the BCIP-NBT reagents, as described in the Materials and Methods.