Fig. 2.

The accumulation of STING in HSV-1(F)–infected Vero cells is independent of protein synthesis. Vero cells seeded in 6-well plates were either mock-infected or exposed to HSV-1(F) virus propagated in HEp-2 cells at 10/pfu/cell (Left) or at 50 pfu/cell (Right). Cycloheximide (100 μg/mL) was added to the cultures the time of exposure to the virus. The cells were harvested at 0, 1, 3, or 7 h after exposure to the virus. Protein analyses were done as described in Fig. 1.