Fig. 3.

Accumulation of STING in newly infected Vero cells reflects the abundance of the protein in which the virus inoculum was made. Vero cells were either mock-infected (lane 1) or exposed to (lane 2) HSV-1(F) (10 pfu/cell) virus stock made in HEp-2 cells or to (lanes 3 and 4) virus that was propagated in HEp-2 followed by passages in Vero, or to (lane 5) virus that was propagated in Vero followed by passage in HEL cells. The cells were harvested at 6 h after exposure to the virus, and equal amounts of total proteins were analyzed as described in Fig. 1.