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Immunoprecipitation of STING with CD9 antibody from Dextran 10 gradient-purified preparations. Dextran 10 gradient-purified fraction containing virions was diluted in 300 μL PBS and reacted with the CD9 rabbit polyclonal antibody. The immunoprecipitated proteins and the proteins in the supernatant fluid were separated in an 11% denaturing polyacrylamide gel and transferred to a nitrocellulose sheet, and the proteins were visualized as in Fig. 1.
