Fig. 3.

Interaction of the 3′ Myc enhancer element and the Myc promoter. (A) Schematic of Myc locus and enhancer with superimposed primers used for chromosome conformation capture (3C) assay. (B) The 3C assay in T6E cells. Graphs show the enrichment of PCR products normalized to HindIII-digested and randomly ligated bacterial artificial chromosome (BAC) DNA spanning the region. (C) Short-term Notch inhibition does not affect chromatin looping. T6E cells were treated with DMSO or 1 μM GSI for 6 h. The 3C assay was performed on the Myc promoter and sequences 3′ of the Myc gene body. Positions of primers used in B and C are shown in A. Data were obtained in triplicate in independent experiments; error bars correspond to the SEM.