Fig. 8.

IFN-γ and TNFα production and CD8 effector function are lower in Egr2 CKO than in WT mice after influenza infection. (A–C) Lung cells were stimulated with PMA + ionomycin (PI) or influenza NP peptide for 5 h, with Golgi-stop added during the last 3 h. Representative flow cytometric profiles for TNFα and IFN-γ expression at the peak response for primary (A) and recall (B) infection models. (C) Absolute number of TNFα⁺IFN-γ⁺CD8⁺ T cells with NP peptide stimulation. (D and E) ChIP-Seq analysis of the EGR2 binding at the Ifng (D) and Tnf (E) loci. (F) mRNA expression (RT-PCR) of Gzmb and Prf1 in CD8⁺ T cells purified after primary or recall infection models. Data are from one representative of three experiments in A and B, from three experiments with 15 mice combined (mean ± SD) in C, representative of two independent experiments in D and E or from three experiments each with five mice per group (F).