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. 2014 Nov 3;111(46):E5007–E5015. doi: 10.1073/pnas.1413210111

Fig. 1.

Fig. 1.

CB2 mRNA expression in WT, CB1−/−, and CB2−/− mice. (A) Mouse CB2 genomic structure and transcripts (mRNAs), illustrating that the CB2 gene contains three exons with two separate promoters (P1 and P2). (B) CB2A and CB2B transcripts and the binding sites of three TaqMan probes used to detect CB2 mRNA by RT-PCR. The CB2A and CB2B probes target the 5′ UTR, whereas the CB2-KO probe targets the CB2-deleted gene sequence in the Zimmer strain of CB2−/− mice. (C) CB2 mRNA was detectable in WT, CB1−/−, and CB2−/− mice when using the CB2A or CB2B probe, but was not detectable in CB2−/− mice when using the CB2-KO probe. The CB2 mRNA levels in each brain or spleen tissue are the relative levels (folds) compared with those in cortex of WT mice (defined as 1). All quantificated data are normalized to control (cortex). Error bars indicate ±SEM. *P < 0.05, compared with WT mice. NM_009924.2 and AK036658.1 are the GenBank cDNA codes.